I have two questions:

QUESTION 1. In metagenomeSeq:

# Calculate proper percentile to normalize data

percentile = cumNormStatFast(genusObj, pFlag = FALSE, rel = 0.1)

percentile

genusObj = cumNorm(genusObj, p = percentile)

genusObj

**If I substitute p = percentile by p = 1 , will metagenomeSeq perform total sum normalization? **

QUESTION 2:

Is it possible to conduct DESeq and TMM normalization using metagenomeSeq?

Many thanks in advance!